Description
Secondary Structure Determination in Proteins using FT-IR
Main applications for the CONFOCHECK are the detection and quantification of conformational changes in proteins and the protein concentration determination. Quantitative determination of the protein concentration and the secondary structure elements (alpha-helix, beta-sheet) from the analyzed protein samples is accomplished within seconds via internal calibrations.
The CONFOCHECK is equipped with several specific protein sampling cuvettes that allow the measurement of solved and membrane bound proteins, even at very low amounts (10 µg). To reach optimal temperature constancy and to facilitate the measurement of temperature induced protein conformational changes, the sample cuvettes are temperature-controlled by a thermostat.
FT-IR Protein Applications:
- Protein quantification
- Detection of conformational changes
- Protein dynamics (temperature induced conformational changes)
- Monitoring of conformational changes during protein aggregation, precipitation and crystallization
- Determination of the secondary structure
- Quantification of all kind of solutes in aqueous samples (buffers, excipients, detergents)
Analyzing Proteins in Pharma:
- Pre-formulation and formulation development: Analysis of the influence of the formulation on protein stability
- Stability studies (stress tests): Influence of pH, temperature, mutation, etc. on the protein stability
- Quality control: Check of protein concentration, structure on correct product composition (buffer, excipients) in production environment
- Binding studies: Analysis of the impact of ligand-binding on the protein structure
AquaSpec Transmission Cell
The AquaSpec™ is used for the measurement of water soluble proteins or solubilized membrane-bound proteins. Using the internal calibrations of the CONFOCHECK the secondary structure and concentration of the analyzed protein are determined directly after measurement (ca. 30 sec).
Applications
- Determination of changes in protein structure
- Protein concentration determination
- Quality control of biopharmaceuticals
- Secondary structure determination
- Quantification of various substances in aqueous solutions
BioATR II
The BioATR II is a micro-ATR unit that was specially developed for the investigation of temperature-induced unfolding, refolding and denaturation processes of proteins in aqueous media.
Applications:
- Automated temperature ramps (0-95°C):
- Analysis of temperature-induced conformational changes
- Measurement of phase transitions in lipids
- Quality control of biopharmaceuticals
- Membrane-standing proteins
- Conformational changes induced by ligand-binding, pH-changes, salts
